Molecular cloning and characterization of a surface-localized adhesion protein in Mycoplasma bovis Hubei-1 strain.

Molecular cloning and characterization of a surface-localized adhesion protein in Mycoplasma bovis Hubei-1 strain.

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Mycoplasma bovis (M.bovis) is an important pathogen that causes here various bovine diseases, such as mastitis in cows and pneumonia in calves.The surface proteins are generally thought to play a central role in the pathogenesis of this organism.

We screened the entire genome of M.bovis Hubei-1 and discovered a gene named vpmaX that encodes the 25 kDa variable surface lipoprotein A (VpmaX).Sequence analysis revealed that VpmaX contains several repetitive units and a typical bacterial lipoprotein signal sequence.

The vpmaX gene was cloned and expressed in E.coli to obtain recombinant VpmaX (rVpmaX).Western blot analysis using a rabbit antibody against rVpmaX demonstrated that VpmaX is a here membrane protein.

Immunostaining visualized via confocal laser scanning microscopy showed that rVpmaX was able to adhere to embryonic bovine lung cells (EBL), and this was also confirmed by a sandwich ELISA.In summary, a surface-localized adhesion protein was identified in M.bovis Hubei-1.